Principle of the method

Identification & Ligation

The probe arms will be ligated together only if they match exactly to the template. A single nucleotide mismatch will not result in ligation of the probe arms.

Amplification

Only ligated probes are amplified.

Hybridization & Detection

The different probes will hybridize to different positions on the microarray. Hybridized amplification products are visualized by colorimetric detection. An image of the microarray, contained in a so called Array Tube, is generated using the Check-Points Tube Reader.